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KMID : 0903519800230010052
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
1980 Volume.23 No. 1 p.52 ~ p.57
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The Mode of Action and the Positional Specificity of Trichosporon cutaneum Lipase
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Abstract
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This study was conducted to clarify the mode of action and the positional specificity of Trichosporon cutaneum lipase during the course of hydrolysis of triolein and monoolein mixture by thin-layer chromatography.
1. The hydrolytic activity of the lipase to oleyl glycerides was in the order of triolein$gt; diolein$gt;monoolein.
2. Both of triolein and diolein were hydrolyzed by the lipase at high and almost the same rate.
3. The hydrolysis of monoolein by the lipase was very slow compared to the other two oleyl glycerides.
4. This lipase appeared to have a very low specificity toward the outer chains of triolein.
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